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Reprint of: Tagging for Protein Expression
1Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, Miami, Florida, USA.
Protein Expression and Purification
|September 6, 2011
Summary
Researchers often use protein tags for recombinant protein expression, improving solubility and purification. This review surveys common tags, aiding scientists in selecting the best options for their protein production needs.
Area of Science:
- Biochemistry
- Molecular Biology
- Protein Expression
Background:
- Protein tags are crucial for recombinant protein production.
- Tags enhance protein solubility and facilitate affinity purification.
- A wide array of tags are available, posing a selection challenge for researchers.
Purpose of the Study:
- To survey commonly used affinity and solubility tags for recombinant protein expression.
- To provide guidance on the selection and application of these protein tags.
- To assist researchers in optimizing their protein production strategies.
Main Methods:
- Literature review of established and novel protein tags.
- Categorization of tags based on function (affinity, solubility).
- Analysis of tag characteristics, advantages, and limitations.
Main Results:
- Overview of popular affinity tags (e.g., His-tag, GST-tag).
- Discussion of solubility-enhancing tags (e.g., MBP, SUMO).
- Comparative analysis to guide tag selection based on experimental goals.
Conclusions:
- Strategic selection of protein tags is vital for successful recombinant protein expression.
- Understanding tag properties aids in overcoming challenges in solubility and purification.
- This survey provides a valuable resource for researchers designing protein expression constructs.
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