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Updated: May 29, 2026
Detection of DNA Double-Stranded Breaks in Mouse Oocytes
Published on: June 23, 2023
Assessment of 1,2-propanediol (PrOH) genotoxicity on mouse oocytes by comet assay
Anais Berthelot-Ricou1, Jeanne Perrin, Carole di Giorgio
1Laboratoire de Biogénotoxicologie et Mutagenèse Environnementale, Fédération de Recherche CNRS 3098 ECCOREV, Facultés de Médecine et Pharmacie, Université de Méditerranée, Marseille, France.
Objective:
To assess the genotoxicity of 1,2-propanediol (PrOH) on mouse oocytes by comet assay.
Design:
In vitro assay using murine model.
Setting:
Biogenotoxicology research laboratory.
Animal(S):
CD1 female mice.
Intervention(S):
Three 40-oocyte groups were exposed to different PrOH concentrations (5%, 7.5%, and 15%). Each concentration was tested during both long and short exposures (1-2 hours and 1-5 minutes) in comparison with control groups. DNA damage was evaluated by a single-cell gel electrophoresis assay, also called "comet assay," and analyzed with Komet software.
Main Outcome Measure(S):
DNA damage was quantified as Olive tail moment (OTM). Interpretation was done on OTM with the use of χ(2).
Result(S):
High PrOH concentrations (7.5% and 15%) induced significant DNA damage on mouse oocytes. The OTM χ(2) values were 4.16 ± 0.40 and 6.80 ± 0.4 with 7.5% PrOH at 1 and 2 hours, respectively, 24.35 ± 1.60 with 15% at 1 hour, and for 2h at 15% the DNA damage was too drastic to calculate OTM χ(2). After 1 and 5 minutes, the OTM χ(2) values were, respectively, 5.19 ± 0.26 and 6.06 ± 0.42 with 7.5%, and 7.53 ± 0.33 and 16.81 ± 0.67 with 15%.
Conclusion(S):
High concentrations of PrOH (7.5% and 15%) induced significant DNA damage on mouse oocytes, whatever the exposure duration. These results should be interpreted with caution, because additional data are needed to evaluate PrOH genotoxicity and DNA oocyte reparation after exposure to high PrOH concentrations.
