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Determination of bovine rotavirus G serotype by polymerase chain reaction

Anjan Mondal1, Ranjit Aich, Sharmila Majee

  • 1Department of Microbiology, West Bengal University of Animal and Fishery Sciences, Belgachia, Kolkata 700037, India. dranjanmondal@gmail.com

Tropical Animal Health and Production
|September 6, 2011
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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...