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Updated: May 29, 2026

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
Published on: March 13, 2014
Gel-electrophoresis and subsequent optical emission (15)n analysis to identify (15)n-labelled protein fractions
1a Department of Chemistry and Biochemistry , University of Agricultural Sciences , Gödöllõ , Hungary.
Abstract:
Abstract A combined procedure to detect of (15)N/(14)N isotope ratios by emission spectrometric analysis after starch gel-electrophoresis was developed. (15)N-labelled proteins of human serum were used to optimise this method. Electrophorised gel slices with protein fractions were directly digested for subsequent isotope analysis. This method is proposed for use in routine analysis for clinical application.
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