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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: May 29, 2026

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Published on: August 15, 2013

Whole cell microtubule analysis by flow cytometry.

Karen C Morrison¹, Paul J Hergenrother

¹Roger Adams Laboratory, Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

Analytical Biochemistry

|September 7, 2011

Summary

This study introduces a rapid flow cytometry method to measure tubulin polymerization in whole cells. This technique efficiently screens anticancer drugs affecting microtubule dynamics.

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Area of Science:

Cell Biology
Pharmacology
Biochemistry

Background:

Microtubule dynamics are crucial for cell function and a key target for anticancer drugs.
Current methods for assessing compounds that affect tubulin polymerization are often inefficient and costly.
Developing high-throughput assays is essential for identifying novel anticancer agents.

Purpose of the Study:

To develop and validate a rapid, quantitative flow cytometry-based method for measuring tubulin polymerization in intact cells.
To utilize this assay for the high-throughput screening and evaluation of small molecules that modulate microtubule dynamics.
To provide a cost-effective and convenient alternative to existing in vitro and low-throughput methods.

Main Methods:

Utilized flow cytometry to quantify the extent of tubulin polymerization within whole cells.
Applied the developed method to assess compounds known to either stabilize or destabilize microtubule formation.
Compared the efficiency and cost-effectiveness against traditional tubulin polymerization assays.

Main Results:

Successfully established a flow cytometry assay for rapid quantification of cellular tubulin polymerization.
Demonstrated the assay's capability to differentiate between microtubule-stabilizing and -destabilizing agents.
Showcased the method's utility for cost-effective, quantitative comparison of small molecules impacting tubulin dynamics.

Conclusions:

The developed flow cytometry method offers a facile and efficient approach to study tubulin polymerization in whole cells.
This technique facilitates rapid screening and comparative analysis of potential anticancer compounds targeting microtubule dynamics.
This assay represents a significant advancement for drug discovery and development in oncology.