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Nitropeptide Profiling and Identification Illustrated by Angiotensin II
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Protein tyrosine nitration in the cell cycle.

Min Jia1, Claudia Mateoiu, Serhiy Souchelnytskyi

  • 1Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden. min.jia@ki.se

Biochemical and Biophysical Research Communications
|September 7, 2011
PubMed
Summary

Protein tyrosine nitration, a key stress response, varies across the cell cycle. This study identifies specific nitrated proteins in G0/G1, S, and G2/M phases, revealing potential regulators of cell proliferation.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Proteomics

Background:

  • Tyrosine nitration is a post-translational modification linked to oxidative and nitrosative stress.
  • Its role in regulating protein function and its abundance throughout the cell cycle are not well understood.
  • Understanding tyrosine nitration patterns is crucial for deciphering cellular stress responses and cell cycle control.

Purpose of the Study:

  • To identify and quantify proteins undergoing tyrosine nitration during different phases of the cell cycle.
  • To investigate the potential impact of cell cycle-specific tyrosine nitration on protein function and cellular processes.
  • To determine if identified nitrated proteins are involved in cell cycle regulation and proliferation.

Main Methods:

  • Synchronization of cells into distinct cell cycle phases (G0/G1, S, G2/M).
  • Proteomic analysis to identify tyrosine-nitrated proteins in each synchronized cell population.
  • Bioinformatic analysis to categorize identified proteins and assess their known functions.

Main Results:

  • Identification of 27 tyrosine-nitrated proteins in G0/G1 phase cells.
  • Identification of 37 tyrosine-nitrated proteins in S phase cells.
  • Identification of 12 tyrosine-nitrated proteins in G2/M phase cells.
  • Nineteen of the identified proteins are known regulators of cell proliferation.

Conclusions:

  • Protein tyrosine nitration levels and targets differ significantly across the cell cycle.
  • The identified tyrosine-nitrated proteins, particularly those involved in proliferation, suggest a role for nitration in cell cycle regulation.
  • This study provides a foundation for further research into the functional consequences of tyrosine nitration in cell cycle control.