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Enumerating bacterial cells on bioadhesive coated slides.

Rima B Franklin¹, Alaina H Campbell, Colleen B Higgins

¹Department of Biology, Virginia Commonwealth University, Richmond, VA 23284-2012, USA.

Journal of Microbiological Methods

|September 7, 2011

Summary

Bioadhesive slides offer a precise and rapid method for enumerating bacteria, outperforming traditional filtration and flow cytometry. While effective for bacterial counts, some slide types may affect cell viability for specific applications.

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Area of Science:

Microbiology
Environmental Science
Biotechnology

Background:

Accurate quantification of bacterial abundance and biomass is crucial for microbiological research.
Conventional methods like epifluorescence microscopy with polycarbonate filters are standard but can be labor-intensive.
Alternative enumeration techniques are needed to improve efficiency and accuracy.

Purpose of the Study:

To evaluate the efficacy of bioadhesive slides as an alternative to standard filtration for bacterial enumeration.
To compare the accuracy and precision of bioadhesive slides with filtration and flow cytometry methods.
To assess the impact of bioadhesive slides on bacterial cell viability.

Main Methods:

Bacterial suspensions from pure cultures, mixed cultures, and environmental samples were stained (acridine orange, Live/Dead®).

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Enumeration was performed using bioadhesive slides, polycarbonate membrane filters, and flow cytometry.

Statistical analysis, including correlation (r) and p-values, was used for comparison.

Main Results:

Bioadhesive slides demonstrated high accuracy and precision, correlating well with standard methods (0.81≤r≤0.95).
Counts from bioadhesive slides were significantly higher than those from polycarbonate filters and flow cytometry.
Certain bioadhesive slides caused significant cell mortality, limiting their use for viability studies.

Conclusions:

Bioadhesive slides provide a highly precise and efficient platform for bacterial enumeration across various concentrations.
This method enhances throughput and allows for the observation of cells less discernible on filters.
While effective for cell counting, potential impacts on cell viability must be considered for specific applications.