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High-throughput Purification of Affinity-tagged Recombinant Proteins
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High-throughput Purification of Affinity-tagged Recombinant Proteins

Published on: August 26, 2012

Reprint of: Purification of Proteins Using Polyhistidine Affinity Tags

By Joshua A Bornhorst, Joseph J Falke

    Protein Expression and Purification
    |September 7, 2011
    PubMed
    Abstract

    No abstract available in PubMed .

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    Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...

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