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Bioassay-directed isolation of active compounds with antiyeast activity from a Cassia fistula seed extract
Subramanion L Jothy1, Zuraini Zakaria, Yeng Chen
1Biological Program, School of Distance Education, Universiti Sains Malaysia, Minden 11800, Penang, Malaysia.
Background And Objective:
Cassia fistula L belongs to the family Leguminosae, and it is one of the most popular herbal products in tropical countries. C. fistula seeds have been used as a herbal medicine and have pharmacological activity which includes anti-bacterial, anti-fungal, and antioxidant properties. The goal of this study was to identify compounds from C. fistula seeds which are responsible for anti-Candida albicans activity using bioassay-directed isolation.
Results:
The preliminary phytochemical screening of the plant seed revealed the presence of anthraquinones, flavonoids, saponins, tannins and terpenoids. The isolation of active compounds was carried out in four steps: multiple extractions, fractionation using column chromatography and purification using preparative thin-layer chromatography (TLC) and liquid chromatography/mass spectrometry (LC/MS). The structure of separated compounds was determined on the basis of mass spectrometry data. One compound was identified is roseanone.
Conclusions:
The MS analysis on the active fraction from seed extract of C. fistula confirmed the presence of roseanone with antiyeast activity.
