Related Experiment Video

Updated: Aug 19, 2026

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
10:03

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Published on: June 27, 2014

Spectral evidence for tyrosine ionization linked to a conformational change in liver alcohol dehydrogenase ternary

W R Laws, J D Shore

    The Journal of Biological Chemistry
    |April 25, 1979
    PubMed

    More Related Videos

    Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics09:18

    Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

    Published on: April 17, 2017

    Preparation of SNS Cobalt(II) Pincer Model Complexes of Liver Alcohol Dehydrogenase06:31

    Preparation of SNS Cobalt(II) Pincer Model Complexes of Liver Alcohol Dehydrogenase

    Published on: March 19, 2020

    Related Concept Videos

    ¹H NMR of Labile Protons: Temporal Resolution01:10

    ¹H NMR of Labile Protons: Temporal Resolution

    Protons bonded to heteroatoms such as nitrogen and oxygen exhibit a range of chemical shift values. This is due to the varying degree of hydrogen bonding between the proton and the heteroatom in other molecules. The extent of hydrogen bonding affects the electron density around the proton, thereby giving different chemical shift values for the protons in the proton NMR spectrum.
    The –OH proton in alcohols typically appears in the range of δ 2 to 5 ppm but can vary depending on the specific...
    ¹H NMR of Labile Protons: Deuterium (²H) Substitution00:48

    ¹H NMR of Labile Protons: Deuterium (²H) Substitution

    This lesson illustrates the role of deuterium substitution in simplifying the NMR spectrum of compounds comprising labile protons. One method employed is the use of deuterium. Amongst the three isotopes of hydrogen, deuterium (2H) has a nucleus composed of one proton and one neutron. When the D2O solvent is added to a pure dry ethanol solution, its labile proton is substituted with deuterium.
    IR Spectrum Peak Broadening: Hydrogen Bonding01:23

    IR Spectrum Peak Broadening: Hydrogen Bonding

    The vibrational frequency of a bond is directly proportional to its bond strength. As a result, stronger bonds vibrate at higher frequencies, while weaker bonds vibrate at lower frequencies. The stretching vibration of the strong O–H bond in alcohols and phenols (very dilute solution or gas phase) appears as a sharp peak at 3600–3650 cm−1.
    However, the extent of hydrogen bonding influences the observed stretching frequency and band broadening. Intermolecular or intramolecular hydrogen bonding...
    IR and UV–Vis Spectroscopy of Aldehydes and Ketones01:29

    IR and UV–Vis Spectroscopy of Aldehydes and Ketones

    Infrared spectroscopy, also known as vibrational spectroscopy, is mainly used to determine the types of bonds and functional groups in molecules. In aldehydes and ketones, the carbonyl (C=O) bond shows an absorption around 1710 cm-1. The C=O bond vibration of an aldehyde occurs at lower frequencies than that of a ketone. In addition to the C=O absorption in an aldehyde, the aldehydic C–H bond also gives two peaks in the 2700–2800 cm-1 range. This absorption, coupled with the C=O stretching, is...
    NMR Spectroscopy and Mass Spectrometry of Aldehydes and Ketones01:15

    NMR Spectroscopy and Mass Spectrometry of Aldehydes and Ketones

    In aldehydes, the hydrogen atom connected to the carbonyl carbon helps distinguish aldehydes from other carbonyl compounds using ¹H NMR spectroscopy. The closeness of aldehydic hydrogen to the electrophilic carbonyl carbon highly deshields the hydrogen atom causing its signal to appear around 10 ppm in the ¹H NMR spectra. α hydrogens split the aldehydic proton signal, which helps identify the number of α hydrogens in the molecule. For instance, one α hydrogen creates a doublet for an aldehydic...
    Mass Spectrometry: Alcohol Fragmentation01:03

    Mass Spectrometry: Alcohol Fragmentation

    Alcohols (R-OH) ionize to lose one non-bonded electron from the oxygen atom, forming molecular ions. Due to their tendency to fragment rapidly, the intensity of the molecular ion peak in the mass spectrum is weak or sometimes absent. The fragmentation patterns for alcohols occur in two ways, i.e. ⍺-cleavage and dehydration. During ⍺-cleavage, the bond at the ⍺-position adjacent to the hydroxyl group cleaves to give a resonance-stabilized cation and a radical. However, intramolecular dehydration...