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Updated: Aug 2, 2026

Generation of Human Kidney Tubuloids from Tissue and Urine
Published on: April 16, 2021
Erythropoietin production in kidney tubular cells
A P Maxwell1, T R Lappin, C F Johnston
1Department of Nephrology, Belfast City Hospital, Northern Ireland.
Abstract:
The erythropoietin gene has been cloned in three mammalian species including man and recombinant erythropoietin is now used to treat the anaemia of chronic renal failure. Despite the isolation of the gene the precise cellular location of erythropoietin synthesis remains controversial. We present studies which demonstrate erythropoietin production by kidney tubular cells. Erythropoietin gene expression (messenger RNA) was detected by in situ hybridization using an oligonucleotide gene probe and the translated protein product by immunohistochemistry employing antibodies raised to pure recombinant DNA derived erythropoietin.
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