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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

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Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers
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Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Published on: August 31, 2021

Dual-trap optical tweezers with real-time force clamp control.

Anders E Wallin1, Heikki Ojala, Gabija Ziedaite

  • 1Department of Physics, University of Helsinki, Helsinki, Finland. anders.wallin@helsinki.fi

The Review of Scientific Instruments
|September 8, 2011
PubMed
Summary

We developed a new dual-trap optical tweezers instrument for real-time force clamp control, enabling precise measurements of molecular forces. This advanced tool is suitable for single molecule biology experiments, offering insights into enzyme and motor functions.

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Last Updated: May 29, 2026

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers
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Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies
06:53

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Published on: November 18, 2022

Area of Science:

  • Biophysics
  • Biochemistry
  • Molecular Biology

Background:

  • Single molecule force clamp experiments are crucial for understanding molecular mechanisms like enzymes and motors.
  • Optical tweezers are a key technology for these studies, allowing precise force application.

Purpose of the Study:

  • To develop a dual-trap optical tweezers instrument with real-time force clamp control.
  • To present a model for force clamp experiments in dumbbell geometry.
  • To validate the instrument's suitability for single molecule biology.

Main Methods:

  • Developed a dual-trap optical tweezers instrument with a 200 kHz update rate for force clamp control (0-100 pN).
  • Created a theoretical model for force clamp experiments in dumbbell geometry.
  • Performed a proof-of-principle experiment using lambda exonuclease on a DNA tether.

Main Results:

  • Observed good agreement between predicted and observed power spectra of bead position and force fluctuations.
  • The developed model accurately predicts and optimizes instrument dynamics.
  • Demonstrated the instrument's capability in a DNA unwinding experiment with lambda exonuclease.

Conclusions:

  • The developed dual-trap optical tweezers instrument provides precise real-time force clamp control.
  • The accompanying model aids in predicting and optimizing instrument performance.
  • The instrument is well-suited for various single molecule biology applications.