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Updated: May 29, 2026

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
Published on: July 25, 2022
Transverse chemical interface detection with coherent anti-Stokes Raman scattering microscopy
Sophie Brustlein1, David Gachet, Franck Billard
1Aix-Marseille Université, Domaine Universitaire Saint-Jéro^me, Institut Fresnel, Mosaic group, CNRS UMR 6133, École Centrale de Marseille, 13397 Marseille cedex 20, France.
Abstract:
Transverse "chemical" interfaces are revealed with a conventional two beam narrowband coherent anti-Stokes Raman scattering microscopy setup in a collinear configuration. The exciting "pump" and "Stokes" beams are focused on the sample in two opposite directions. The subtraction of the two generated anti-Stokes signals gives rise to a signal that is directly proportional to the pure Raman spectrum of the resonant medium. This property is used to highlight an interface between glass and N,N-dimethylformamide (DMF) and recover the pure Raman spectrum of DMF around its 1408 cm(-1) vibrational band.
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