Related Experiment Video
Updated: May 29, 2026
06:37
Staphylococcus aureus Growth using Human Hemoglobin as an Iron Source
Published on: February 7, 2013
Hemoglobin Lepore Hollandia in India
A Nadkarni1, K Italia, P Sawant
1National Institute of Immunohaematology (ICMR), Parel, Mumbai, India. anitahnadkarni@yahoo.com
International Journal of Laboratory Hematology
|September 8, 2011
Summary
Hemoglobin Lepore Hollandia, a rare δβ globin chain variant, causes a β thalassemia phenotype. Identification requires combined chromatographic, electrophoretic, and molecular analyses.
Area of Science:
- Hematology
- Molecular Genetics
Background:
- Hemoglobin Lepore is an abnormal hemoglobin with a fused δβ globin chain.
- Heterozygous Hb Lepore presents as β thalassemia with elevated HbF.
Purpose of the Study:
- To identify and characterize Hb Lepore Hollandia in affected families.
- To investigate the clinical phenotype and genetic origin of Hb Lepore Hollandia.
Main Methods:
- High-Performance Liquid Chromatography (HPLC) and DNA analysis were employed.
- Eight individuals from three families were studied, including heterozygotes and a compound heterozygote.
Main Results:
- Seven heterozygotes and one compound heterozygote for Hb Lepore Hollandia were identified.
- Heterozygotes exhibited elevated HbF levels (mean 3.2%).
- The identified genes shared a common β globin cluster haplotype, suggesting a single origin.
Conclusions:
- Hemoglobin Lepore Hollandia is uncommon in the Indian population.
- A combination of chromatographic, electrophoretic, and molecular methods is effective for identification.
Related Concept Videos
01:24Hemoglobin
Hemoglobin is a globular protein made up of four subunits. Two of these subunits are alpha chains, and the other two are beta chains. Each subunit contains a molecule of heme, which has an iron atom and can bind to oxygen. When an oxygen molecule binds to one heme group, it changes the shape of hemoglobin, making it easier for the other heme groups to bind oxygen as well.
When all four heme groups are bound to oxygen, the resulting molecule is called oxyhemoglobin. As a result, arterial blood...
When all four heme groups are bound to oxygen, the resulting molecule is called oxyhemoglobin. As a result, arterial blood...
01:57Gene Families
Gene families consist of groups of genes proposed to have originated from a common ancestor. Typically these arise through events in which a gene or genes are mistakenly duplicated during cell division. Unlike their parent genes (which are subject to selection pressure to maintain function), these gene copies do not need to preserve their sequences and may evolve at a relatively faster rate.
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
01:27Oxygen Transport in the Blood
Hemoglobin (Hb) is a crucial molecule in the human body, consisting of four polypeptide chains, each bound to an iron-containing heme group. This unique structure enables hemoglobin to bind to oxygen, with each molecule capable of combining with four molecules of oxygen, leading to rapid and reversible oxygen loading. When fully loaded with oxygen, it is called oxyhemoglobin, while hemoglobin that has released oxygen is called reduced hemoglobin or deoxyhemoglobin. As hemoglobin binds oxygen,...
02:15Protein and Protein Structure
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme can...
A protein's shape is critical to its function. For example, an enzyme can...
02:21Globular and Fibrous Proteins
Many proteins can be classified into two distinct subtypes - globular or fibrous. These two types differ in their shapes and solubilities.
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
01:19Rh Blood Group
The Rhesus (Rh) antigen is crucial in determining blood groups and ensuring compatibility during blood transfusions.