A microfabricated magnetic force transducer-microaspiration system for studying membrane mechanics

The application of forces to cell membranes is a powerful method for studying membrane mechanics. To apply controlled dynamic forces on the piconewton scale, we designed and characterized a microfabricated magnetic force transducer (MMFT) consisting of current-carrying gold wires patterned on a sapphire substrate. The experimentally measured forces applied to paramagnetic and ferromagnetic beads as a function of applied current agree well with theoretical models. We used this device to pull tethers from microaspirated giant unilamellar vesicles and measure the threshold force for tether formation. In addition, the interlayer drag coefficient of the membrane was determined from the tether-return velocity under magnetic force-free conditions. At high levels of current, vesicles expanded as a result of local temperature changes. A finite element thermal model of the MMFT provided absolute temperature calibration, allowing determination of the thermal expansivity coefficient of stearoyl-oleoyl-phosphatidycholine vesicles (1.7 ± 0.4 × 10(-3) K(-1)) and characterization of the Joule heating associated with current passing through the device. This effect can be used as a sensitive probe of temperature changes on the microscale. These studies establish the MMFT as an effective tool for applying precise forces to membranes at controlled rates and quantitatively studying membrane mechanical and thermo-mechanical properties.