Cytoprotective properties of a fullerene derivative against copper

To delineate the complexity of the response of cells to nanoparticles we have performed a study on HT-29 human colon carcinoma cells exposed first to a fullerene derivative C(60)(OH)(20) and then to physiological copper ions. Our cell viability, proliferation, and intracellular reactive oxygen species (ROS) production assays clearly indicated that C(60)(OH)(20) suppressed cell damage as well as ROS production induced by copper, probably through neutralization of the metal ions by C(60)(OH)(20) in the extracellular space, as well as by adsorption and uptake of the nanoparticles surface-modified by the biomolecular species in the cell medium. This double-exposure study provides new data on the effects of nanoparticles on cell metabolism and may aid the treatment of oxidant-mediated diseases using nanomedicine.