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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

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Related Experiment Video

Updated: May 29, 2026

Micro-Mechanical Characterization of Lung Tissue Using Atomic Force Microscopy
11:10

Micro-Mechanical Characterization of Lung Tissue Using Atomic Force Microscopy

Published on: August 28, 2011

Micro-mechanical characterization of lung tissue using atomic force microscopy.

Fei Liu1, Daniel J Tschumperlin

  • 1Molecular and Integrative Physiological Sciences, Department of Environmental Health, Harvard School of Public Health, USA.

Journal of Visualized Experiments : Jove
|September 8, 2011
PubMed
Summary
This summary is machine-generated.

Researchers developed atomic force microscopy (AFM) methods to measure lung tissue stiffness at the cellular level. This technique maps spatial variations in stiffness, aiding understanding of tissue remodeling in diseases like fibrosis.

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Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy
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Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy

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Related Experiment Videos

Last Updated: May 29, 2026

Micro-Mechanical Characterization of Lung Tissue Using Atomic Force Microscopy
11:10

Micro-Mechanical Characterization of Lung Tissue Using Atomic Force Microscopy

Published on: August 28, 2011

Extracting the Young's Modulus of Native Murine Pulmonary Basement Membranes from Atomic Force Microscopy Derived Force Maps
10:55

Extracting the Young's Modulus of Native Murine Pulmonary Basement Membranes from Atomic Force Microscopy Derived Force Maps

Published on: January 31, 2025

Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy
08:41

Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy

Published on: June 27, 2013

Area of Science:

  • Biophysics
  • Cell Biology
  • Tissue Engineering

Background:

  • Matrix stiffness significantly impacts cell behavior and tissue function.
  • Spatial variations in tissue stiffness within organs are poorly understood, especially during disease-related remodeling.
  • Accurate, localized stiffness measurements are crucial for understanding cellular physiology in health and disease.

Purpose of the Study:

  • To develop and validate methods for measuring local elastic properties of lung parenchyma at a cellular scale.
  • To map spatial variations in tissue stiffness in fresh murine lung tissue.
  • To correlate mechanical properties with anatomical and pathological features.

Main Methods:

  • Utilized atomic force microscopy (AFM) microindentation on fresh murine lung tissue.
  • Employed specific AFM indentors, cantilevers, and indentation depths for shear modulus measurements.
  • Performed systematic tissue sampling to create mechanical property maps, integrating with phase contrast and fluorescence imaging.

Main Results:

  • Successfully measured local shear modulus of lung parenchyma at a cellular-relevant spatial scale.
  • Generated maps revealing significant local spatial variations in tissue stiffness.
  • Demonstrated correlations between measured stiffness and matrix deposition, particularly in fibrotic tissue.

Conclusions:

  • The developed AFM microindentation method accurately characterizes local lung tissue stiffness.
  • Spatial variations in stiffness are significant and linked to tissue composition and pathology.
  • This approach is extendable to other soft tissues and diseases for comprehensive mechanical profiling.