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Protein coverage on polymer nanolayers leading to mesenchymal stem cell patterning.

Jungmok You1, Akihito Yoshida, June Seok Heo

  • 1Department of Chemical and Biomolecular Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Korea.

Physical Chemistry Chemical Physics : PCCP
|September 8, 2011
PubMed
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UV light alters diphenylamino-s-triazine bridged p-phenylene vinylene polymer (DTOPV) nanofilms, enhancing gelatin adsorption and promoting mesenchymal stem cell (MSC) attachment for biomaterial applications.

Area of Science:

  • Materials Science
  • Biomaterials Engineering
  • Surface Chemistry

Background:

  • Understanding protein-surface interactions is crucial for developing advanced biomaterials.
  • Functional polymer nanolayers offer tunable properties for biomedical applications.
  • Controlling protein adsorption influences cell behavior on material surfaces.

Purpose of the Study:

  • To investigate the interaction of gelatin and albumin with photo-reactive diphenylamino-s-triazine bridged p-phenylene vinylene polymer (DTOPV) nanofilms.
  • To explore how UV exposure affects protein adsorption and subsequent mesenchymal stem cell (MSC) attachment on DTOPV.
  • To determine the influence of protein concentration on selective adsorption and cell patterning.

Main Methods:

  • Surface Plasmon Resonance (SPR) spectroscopy was employed to quantify protein adsorption.

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  • DTOPV nanofilms were exposed to UV light to induce structural changes.
  • Mesenchymal stem cells (MSCs) were cultured on modified DTOPV surfaces to assess attachment and patterning.

    • Main Results:

    • UV exposure significantly increased gelatin adsorption on DTOPV nanofilms.
    • Albumin adsorption decreased upon UV exposure of DTOPV.
    • Selective protein adsorption was concentration-dependent, with highest selectivity at low protein concentrations (3.5 μg ml⁻¹).
    • Selective MSC attachment correlated with selective protein adsorption, enabling successful MSC patterning on UV-treated DTOPV films.

    Conclusions:

    • UV-induced changes in DTOPV nanofilms modulate protein adsorption, favoring gelatin.
    • Controlled gelatin adsorption promotes selective MSC attachment and patterning.
    • This study provides insights into biomolecule-surface interactions for designing biocompatible materials for cell engineering and medical implants.