Related Experiment Video

Updated: May 29, 2026

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
08:40

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Published on: April 8, 2016

Immunohistochemical and immunofluorescence procedures for protein analysis

Kishore Reddy Katikireddy1, Finbarr O'Sullivan

  • 1National Institute of Cellular Biotechnology, Dublin City University, Ireland. kishore.katikireddy2@mail.dcu.ie

Methods in Molecular Biology (Clifton, N.J.)
|September 8, 2011
PubMed
Summary

Immunohistochemistry (IHC) and immunofluorescence (IF) are vital diagnostic tools that visualize antibodies binding to cellular antigens. Proper tissue fixation and staining methods are crucial for accurate protein localization and avoiding artifacts in IHC and IF.

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