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Structure-functions of HspB1 (Hsp27)
1Stress, Chaperons, and Cell Death Laboratory, CNRS UMR 5534, Claude Bernard University Lyon 1, Villeurbanne, France. arrigo@univ-lyon1.fr
Abstract:
Human HspB1 (also denoted Hsp27) is a well-known member, together with alphaB-crystallin, of the small heat-shock (or stress) proteins (sHsps) (20-40 kDa). In this chapter, I describe procedures for testing the oligomeric and phosphorylation patterns of HspB1 as well as its interaction with specific partner/client polypeptides using tissue culture cells genetically modified to express different levels of this protein. The procedures have been developed in my laboratory and could be used in any well-established cellular laboratory. In addition, the different procedures presented here could be extended to test the nine other human sHsp members as well as sHsps from other species.
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