Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nitric Oxide Signaling Pathway01:28

Nitric Oxide Signaling Pathway

Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure to...
Key Elements for Plant Nutrition02:35

Key Elements for Plant Nutrition

Like all living organisms, plants require organic and inorganic nutrients to survive, reproduce, grow and maintain homeostasis. To identify nutrients that are essential for plant functioning, researchers have leveraged a technique called hydroponics. In hydroponic culture systems, plants are grown—without soil—in water-based solutions containing nutrients. At least 17 nutrients have been identified as essential elements required by plants. Plants acquire these elements from the atmosphere, the...
Seed Structure and Early Development of the Sporophyte02:33

Seed Structure and Early Development of the Sporophyte

Seed structures are composed of a protective seed coat surrounding a plant embryo, and a food store for the developing embryo. The embryo contains the precursor tissues for leaves, stem, and roots. The endosperm and cotyledons—seed leaves—act as the food reserves for the growing embryo.
Mechanism of Angiogenesis01:10

Mechanism of Angiogenesis

Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Thiamin addition to soil increases potato tuber thiamin content under greenhouse conditions.

PeerJ·2026
Same author

Potato dihaploids uncover diverse alleles to facilitate diploid potato breeding.

The plant genome·2026
Same author

The structure and allelic diversity of the self-incompatibility locus (S-locus) in diploid potatoes inferred from genome sequences and transcriptome data from styles and pollen.

The plant genome·2025
Same author

Detection and Quantification of Potato Spindle Tuber Viroid in Wild and Cultivated Potatoes Using a New Validated RT-qPCR Method.

Plant disease·2025
Same author

Uncovering pre-cytokinetic block in cancer cells under shear stress using a disturbed flow-generating device.

Scientific reports·2025
Same author

A collateral circulation in ischemic stroke accelerates recanalization due to lower clot compaction.

PloS one·2024

Related Experiment Video

Updated: May 29, 2026

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
08:32

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Published on: March 16, 2017

Nitric oxide methods in seed biology.

Paul C Bethke1, Igor G L Libourel, Jan Vitecek

  • 1Department of Horticulture, University of Wisconsin-Madison, Madison, WI, USA. paul.bethke@ars.usda.gov

Methods in Molecular Biology (Clifton, N.J.)
|September 8, 2011
PubMed
Summary

Nitric oxide (NO) is vital for seed biology but unstable in air. This chapter details methods for applying NO gas or donors, removing NO, and detecting it using probes and specialized apparatus.

More Related Videos

Analytical Techniques for Assaying Nitric Oxide Bioactivity
11:28

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
08:58

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

Published on: February 25, 2016

Related Experiment Videos

Last Updated: May 29, 2026

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
08:32

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Published on: March 16, 2017

Analytical Techniques for Assaying Nitric Oxide Bioactivity
11:28

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
08:58

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

Published on: February 25, 2016

Area of Science:

  • Plant Biology
  • Biochemistry
  • Chemical Ecology

Background:

  • Nitric oxide (NO) is a crucial signaling molecule in plant systems, particularly in seed biology.
  • The gaseous nature of NO presents experimental challenges due to its rapid conversion to other nitrogen oxides in aerobic conditions.

Purpose of the Study:

  • To provide comprehensive methods for working with nitric oxide in seed biology research.
  • To outline techniques for NO application, scavenging, and detection.

Main Methods:

  • Application of NO as a pure gas.
  • Utilization of NO-donor compounds for controlled NO release.
  • Employing NO scavengers to reduce or remove NO.
  • Detection of NO using fluorescent probes.
  • Description of an apparatus with an oxidizer column for NO management.

Main Results:

  • Established protocols for the controlled application of nitric oxide in experimental settings.
  • Demonstrated effective methods for NO scavenging and reduction.
  • Presented reliable techniques for NO detection in biological samples.

Conclusions:

  • Standardized methods are essential for reproducible research on nitric oxide's role in seed biology.
  • The described techniques facilitate the study of NO's physiological functions in plants.
  • This chapter serves as a practical guide for researchers investigating NO in plant science.