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Related Concept Videos

mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

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Related Experiment Video

Updated: May 29, 2026

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species
12:53

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species

Published on: May 19, 2018

[Relationship between RNA degradation and postmortem interval in mice].

Yi Zhu1, Ying-Chun Dong, Wei-Bo Liang

  • 1West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China. zhuyi0216@gmail.com

Fa Yi Xue Za Zhi
|September 9, 2011
PubMed
Summary
This summary is machine-generated.

Temperature significantly impacts RNA degradation in mouse brains postmortem. 18S ribosomal RNA (rRNA) is more stable than beta-actin mRNA, offering a reliable marker for estimating the postmortem interval (PMI).

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Last Updated: May 29, 2026

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species
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Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species

Published on: May 19, 2018

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Published on: January 17, 2019

Area of Science:

  • Molecular Biology
  • Forensic Science
  • Biochemistry

Context:

  • Accurate determination of the postmortem interval (PMI) is crucial in forensic investigations.
  • RNA integrity is affected by environmental factors like temperature after death.
  • Beta-actin mRNA and 18S ribosomal RNA (rRNA) are commonly used housekeeping genes.

Purpose:

  • To investigate the degradation patterns of beta-actin mRNA and 18S rRNA in mouse brains at different temperatures and time points.
  • To assess the correlation between RNA degradation and the postmortem interval (PMI).
  • To evaluate the relative stability of beta-actin mRNA and 18S rRNA for PMI estimation.

Summary:

  • Mouse brains were analyzed for beta-actin mRNA and 18S rRNA degradation at 4°C and 37°C over 48 hours postmortem.
  • Real-time PCR quantified RNA levels, revealing faster degradation at 37°C compared to 4°C.
  • 18S rRNA demonstrated greater stability than beta-actin mRNA throughout the study period.

Impact:

  • This research provides a new theoretical foundation for estimating PMI using RNA degradation.
  • The findings suggest that 18S rRNA is a more robust biomarker for PMI estimation than beta-actin mRNA.
  • This study can supplement traditional forensic methods for determining time since death.