[In vitro modeling of cell-scaffold interaction]

The simple approach for modeling of surface ligand - cell receptor interaction is proposed to control the effectiveness of peptide acceptor selected to be immobilized on a scaffold surface in order to promote specific cell adhesion and their subsequent proliferation and bone tissue formation. For experimental realization of such approach the affinity chromatography with use of macroporous monolithic sorbent is suggested. The biospecific GRGDSP-peptide performed the role of scaffold surface ligand which is responsible for cell adhesion, while the "cells" were simulated by polymer (polystyrene) micro particles with EDYPVDIYYLM-DLSYSMKDD-peptide immobilized on their surface. The latter peptide is the integrin molecule active site which is responsible for RGD-sequence binding. Thus the ultra-short monolithic chromatography columns (CIM-disks) represent the simplified model of a scaffold possessing biospecific properties. The qualitative evaluation of complement interaction parameters was performed via frontal analysis method followed by adsorption isotherm plotting and subsequent linearization and mathematical treatment. The data obtained reliably indicate the highly specific character of biological pair binding. This was in a good accordance with results obtained in the cell culture experiments.