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Related Experiment Video

Updated: May 29, 2026

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

Novel strategy for microsphere-mediated DNA transfection.

Jessica G Borger¹, Juan Manuel Cardenas-Maestre, Rose Zamoyska

¹Institute of Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom.

Bioconjugate Chemistry

|September 9, 2011

Summary

Researchers developed a novel microsphere method for delivering plasmid DNA, proving effective for gene delivery in T cells. This noninvasive technique shows promise for hard-to-transfect cells.

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Last Updated: May 29, 2026

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation

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Published on: July 31, 2015

High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology

High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology

Published on: August 8, 2019

Area of Science:

Biotechnology
Molecular Biology
Gene Delivery Systems

Background:

Efficient gene delivery is crucial for research and therapeutic applications.
Current methods face challenges with transfection-resistant and non-dividing cells.

Purpose of the Study:

To develop and evaluate a novel microsphere-mediated gene delivery system.
To assess the efficiency and applicability of this method for plasmid DNA delivery.

Main Methods:

Plasmid DNA was linearized and functionalized via aminomodification.
Functionalized plasmid DNA was conjugated to carboxy-functionalized microspheres.
Transfection efficiency was evaluated in a T cell hybridoma line using YFP expression.

Main Results:

Successful conjugation of plasmid DNA to microspheres was achieved.
Efficient transfection and expression of a Yellow Fluorescent Protein (YFP) fusion protein were observed in T cells.
Microsphere-mediated delivery demonstrated noninvasive, nontoxic, and efficient gene transfer.

Conclusions:

Microsphere-mediated delivery of plasmid DNA is a viable and efficient gene delivery strategy.
This method holds potential for transfecting challenging cell types, including non-dividing primary cells like naïve T cells.