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Related Concept Videos

Sampling Methods: Sample Types01:18

Sampling Methods: Sample Types

Sampling materials are classified into three main types: solid, liquid, and gas.
Solid samples include a variety of substances, such as sediments from water bodies, soil, metals, and biological tissues. Two standard methods for extracting sediments from water bodies are grab sampling and piston coring. Grab sampling involves using a device to collect a discrete sediment sample from the bottom of a water body with minimal disturbance. Grab samples do not always represent the entire area due to...
Sample Preparation for Analysis: Overview01:21

Sample Preparation for Analysis: Overview

Sample preparation is an essential step in the analytical process. It involves preparing a sample so that it can be analyzed accurately. The goal is to extract the analyte, the substance you want to measure, from the sample while removing any components that may interfere with the analysis. Sample preparation techniques vary depending on the physical state of the sample.
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...
DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
Sampling Methods: Overview01:06

Sampling Methods: Overview

A sample refers to a smaller subset representative of a larger population. In analytical chemistry, studying or analyzing an entire population is often impractical or impossible. Therefore, samples are used to draw inferences and generalize the whole population. The sampling method selects individuals or items from a population to create a sample. Standard sampling methods include random, judgemental, systematic, stratified, and cluster sampling. 
In analytical chemistry, the choice of sampling...

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Related Experiment Video

Updated: May 29, 2026

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
11:17

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

Published on: June 1, 2017

Sample treatment based on extraction techniques in biological matrices.

Nadia Y Ashri1, Mohamed Abdel-Rehim

  • 1Najd Consulting Hospital, Riyadh, Kingdom of Saudi Arabia.

Bioanalysis
|September 9, 2011
PubMed
Summary
This summary is machine-generated.

Effective bioanalysis relies on robust sample preparation. This article details high-throughput techniques like protein precipitation and solid-phase extraction crucial for pharmaceutical drug development and analysis.

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Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Published on: July 11, 2014

Area of Science:

  • Analytical Chemistry
  • Pharmaceutical Sciences
  • Biotechnology

Background:

  • Sample preparation is the critical first step in bioanalysis.
  • Efficient methods are essential for accurate and timely results, especially in pharmaceutical research.
  • High-throughput analysis is increasingly vital for processing large numbers of biological samples.

Purpose of the Study:

  • To discuss the concept and strategies of sample preparation in bioanalysis.
  • To highlight the requirements for effective sample preparation.
  • To present widely used and high-throughput sample preparation techniques in the pharmaceutical industry.

Main Methods:

  • Protein precipitation
  • Solid-phase extraction (SPE)
  • Liquid-liquid extraction (LLE)
  • Microextraction techniques
  • Selective extraction using molecular imprinted phases

Main Results:

  • Various sample preparation methods are presented, including precipitation, SPE, LLE, and microextraction.
  • High-throughput techniques are essential for the pharmaceutical industry's need for rapid analysis.
  • Selective extraction methods, such as molecular imprinted phases, offer potential for enhanced specificity.

Conclusions:

  • Sample preparation is fundamental to successful bioanalysis.
  • High-throughput techniques are critical for modern pharmaceutical bioanalysis.
  • Selective extraction methods show promise for future advancements in analyte isolation.