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Related Experiment Videos

6 beta-Hydroxycortisol excretion in hypercortisolemic states.

E Voccia, P Saenger, R E Peterson

    The Journal of Clinical Endocrinology and Metabolism
    |March 1, 1979
    PubMed
    Summary

    Urinary 6beta-hydroxycortisol (6betaOHF) is a more effective diagnostic marker for hypercortisolemia than other cortisol measurements. This study found 6betaOHF excretion reliably indicates conditions like Cushing's syndrome or disease.

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    Area of Science:

    • Endocrinology
    • Clinical Chemistry
    • Pediatric Endocrinology

    Background:

    • Assessing hypercortisolemia relies on measuring urinary cortisol metabolites.
    • Urinary 6beta-hydroxycortisol (6betaOHF) is a metabolite whose diagnostic utility in pediatric hypercortisolemia requires further investigation.

    Purpose of the Study:

    • To compare the diagnostic value of urinary 6betaOHF excretion against free cortisol and 17-hydroxycorticosteroid (17OH) excretion.
    • To evaluate 6betaOHF as a marker for chronic hypercortisolemia in children.

    Main Methods:

    • Measured urinary 6betaOHF, free cortisol, and 17OH in normal children, patients with Cushing's syndrome/disease (CSD), and those on cortisol therapy.
    • Analyzed the ratio of 6betaOHF to 17OH (6betaOHF:17OH) under various conditions, including ACTH stimulation and high-dose cortisol administration.

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    Main Results:

    • Normal pediatric 6betaOHF excretion was established (0.23 +/- 0.03 mg/m2/24 h).
    • Both ACTH infusion and high-dose cortisol normalized the 6betaOHF:17OH ratio, suggesting cortisol induces 6beta-hydroxylase in hypercortisolemic states.
    • The 6betaOHF:17OH ratio was significantly elevated in CSD patients, exceeding the normal range.

    Conclusions:

    • Urinary 6betaOHF excretion is a more sensitive and rapid diagnostic test for chronic hypercortisolemia compared to urinary 17OH or free cortisol.
    • Measurement of urinary 6betaOHF is recommended as a valuable diagnostic tool for hypercortisolemic states.