Studies on human CFU-Mix microvolume culture by use of limiting dilution assay

By use of limiting dilution assay we investigated the bipotent and pluripotent hemopoietic progenitor cells (CFU-Mix) from 21 normal adults, cultured for 32 times. The CFU-Mix counts were 35.7 colonies/10(6) bone marrow cells. According to different cellular elements they could be divided into 5 groups, namely GEMM, GE, GL, GMeg and GM phi. It was suggested that differentiation of the stem cell is of stochastic process, influenced by different hemopoietic growth factors and cellular microenvironment. Our model seems to be suitable for studying differentiation of the stem cells. The rates of 3H-TdR and 55 + 59Fe incorporation were determined, and the 2nd day of culture was found to be the delayed stage of cell growth.