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Related Experiment Video

Updated: May 29, 2026

High-Throughput Capable Three-Dimensional Tissue Model for Quantification of Electroporation Thresholds

High-Throughput Capable Three-Dimensional Tissue Model for Quantification of Electroporation Thresholds

Published on: August 19, 2025

Equivalent pulse parameters for electroporation.

Gorazd Pucihar¹, Jasna Krmelj, Matej Reberšek

¹Faculty of Electrical Engineering, University of Ljubljana, Ljubljana, Slovenia. gorazd.pucihar@fe.uni-lj.si

IEEE Transactions on Bio-Medical Engineering

|September 9, 2011

Summary

This summary is machine-generated.

Electroporation success depends on pulse parameters. Equivalent parameters can be found using mathematical models, enabling flexible treatment planning for applications like electrochemotherapy.

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Area of Science:

Biophysics
Cellular Biology
Biomedical Engineering

Background:

Electroporation requires precise pulse parameters for effective cell treatment.
Deviations from recommended parameters necessitate finding equivalent settings for consistent outcomes.

Purpose of the Study:

To establish mathematical relationships between electroporation pulse amplitude, duration, and number.
To identify equivalent pulse parameters that yield similar cell electroporation fractions.

Main Methods:

Varied pulse durations (150 ns to 100 ms) and pulse numbers (1 to 128).
Utilized Fura 2-AM to quantify cell electroporation via Ca(2+) influx.
Developed and validated a mathematical model for electroporation.

Main Results:

Lower amplitudes are required for longer pulse durations or higher pulse numbers to achieve the same electroporation fraction.
A derived electroporation model accurately described experimental data across a wide range of pulse durations.
Simpler logarithmic or power functions effectively described parameter relationships within specific ranges (e.g., 0.1-100 ms).

Conclusions:

Mathematical models, particularly power and logarithmic functions, are valuable for determining equivalent electroporation parameters.
These findings support optimized treatment planning for electroporation-based therapies, including electrochemotherapy and nonthermal irreversible electroporation.