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Updated: May 29, 2026

The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii
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The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii

Published on: April 18, 2016

Prevalence of Coxiella burnetii in livestock abortion material using PCR

G C Pritchard1, R P Smith, J Errington

  • 1Animal Health and Veterinary Laboratories Agency (AHVLA) - Bury St Edmunds, Rougham Hill, Suffolk IP33 2RX, UK. geoffpritchard@o2.co.uk

The Veterinary Record
|September 9, 2011
PubMed
Abstract

No abstract available in PubMed .

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...