Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
Nucleosome Remodeling02:54

Nucleosome Remodeling

Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
Mutations01:35

Mutations

Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
Mutations01:39

Mutations

Overview
Protein Denaturation01:28

Protein Denaturation

The function of proteins depends on their native three-dimensional structure, which is dictated by the amino acid sequence of the specific protein. Folding of the polypeptide chain takes place under specific conditions that energetically favor the folded conformation. In contrast, protein denaturation occurs spontaneously under unfavorable conditions that disrupt the integrity of the folded conformation. Thus, the chemical and physical environment of a protein, such as significant changes in pH...
¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Uncovering elusive ultrafast charge transfer-driven structural changes in 4,4'-bis(9-carbazol-9-yl)-1,1'-biphenyl, a paradigmatic molecular triad.

Physical chemistry chemical physics : PCCP·2025
Same author

Impact of Hot Carrier Dynamics on Photoelectrocatalytic Activity on Au@Pd Antenna-Reactor Nanoparticles.

Journal of the American Chemical Society·2025
Same author

Time-Resolved Spectroelectrochemical Observation of Overlayer-Induced Charge Carrier Dynamics in Water Photooxidation.

Angewandte Chemie (International ed. in English)·2025
Same author

Structural dynamics of protein-protein association involved in the light-induced transition of Avena sativa LOV2 protein.

Nature communications·2024
Same author

Photoinduced Group Transposition via Iridium-Nitrenoid Leading to Amidative Inner-Sphere Aryl Migration.

Angewandte Chemie (International ed. in English)·2024
Same author

Charge Trapping in Semiconductor Photocatalysts: A Time- and Space-Domain Perspective.

Journal of the American Chemical Society·2024

Related Experiment Video

Updated: May 29, 2026

Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

Conformational changes of non-B DNA.

Jungkweon Choi1, Tetsuro Majima

  • 1The Institute of Scientific and Industrial Research (SANKEN), Osaka University, Ibaraki, Osaka 567-0047, Japan.

Chemical Society Reviews
|September 9, 2011
PubMed
Summary

Non-B DNA structures, unlike standard B-DNA, can form various alternative structures. Understanding their dynamics is crucial for both disease research and biotechnological applications.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • B-DNA is the standard right-handed double helix.
  • Repetitive DNA sequences can form non-B DNA structures (e.g., G-quadruplex, i-motif, Z-DNA) under specific conditions.
  • These non-B DNA structures are linked to genetic instability and human diseases.

Purpose of the Study:

  • To review recent studies on the conformational dynamics of non-B DNA structures.
  • To highlight the importance of understanding non-B DNA dynamics for disease mechanisms and biotechnological applications.

Main Methods:

  • Critical review of existing literature.
  • Focus on conformational dynamics of specific non-B DNA structures.

Main Results:

More Related Videos

High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States
04:37

High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States

Published on: June 29, 2021

Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion
09:17

Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion

Published on: March 1, 2022

Related Experiment Videos

Last Updated: May 29, 2026

Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States
04:37

High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States

Published on: June 29, 2021

Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion
09:17

Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion

Published on: March 1, 2022

  • Non-B DNA structures exhibit diverse conformational dynamics.
  • DNA breakage hotspots are often located near non-B DNA-forming sequences.
  • Non-B DNA structures offer potential for nanotechnology due to their robustness and lack of toxic byproducts.

Conclusions:

  • Understanding non-B DNA structural changes is vital.
  • Non-B DNA holds promise for biotechnology and nanotechnology.
  • Further research into non-B DNA dynamics is warranted.