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Related Experiment Video

Updated: May 29, 2026

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies
10:16

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Published on: September 15, 2016

Optimized autoantibody profiling on protein arrays.

Sara L O'Kane1, John K O'Brien, Dolores J Cahill

  • 1Conway Institute of Biomolecular & Biomedical Research, University College Dublin, Dublin, Ireland.

Methods in Molecular Biology (Clifton, N.J.)
|September 9, 2011
PubMed
Summary

This study introduces a large-scale protein array for identifying autoantibodies (AAb) in human serum. This method aids in discovering novel protein biomarkers for diagnosing and monitoring various diseases, including cancers and autoimmune conditions.

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Area of Science:

  • Biochemistry
  • Immunology
  • Oncology

Background:

  • Autoantibody (AAb) profiling is crucial for diagnosing autoimmune diseases and is emerging in oncology.
  • Protein arrays offer high-throughput screening of serum samples for AAb detection.

Purpose of the Study:

  • To describe the use of a large protein array for identifying autoantibodies in human sera.
  • To identify candidate protein biomarkers for disease diagnosis, staging, progression, and therapy response.

Main Methods:

  • Utilized a protein array with over 10,000 non-redundant human recombinant proteins.
  • Screened a test set of human serum samples to identify antibody-binding proteins.
  • Employed robotic spotting on microarrays for efficient screening.

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Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays
13:22

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

Published on: October 23, 2019

Related Experiment Videos

Last Updated: May 29, 2026

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies
10:16

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Published on: September 15, 2016

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays
13:22

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

Published on: October 23, 2019

Main Results:

  • Identified proteins bound by antibodies in human sera.
  • These identified proteins show potential as candidate biomarkers.

Conclusions:

  • The described protein array approach facilitates the discovery of potential biomarkers.
  • This method supports the development of diagnostic and prognostic tools for various diseases.