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Updated: May 29, 2026

Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction
Published on: December 3, 2017
Is repetitive intraoperative splash basin use a source of bacterial contamination in total joint replacement?
Sergio A Glait1, Ran Schwarzkopf, Steven Gould
1Department of Orthopedic Surgery, NYU Hospital for Joint Diseases, New York, New York, USA.
Abstract:
Splash basins are used in arthroplasty cases to wash instruments. Several studies in the literature have shown these basins being a potential source of bacterial infection. This study assesses the risk of contamination of intraoperative splash basins used to wash and store instruments. A total of 46 random clean primary arthroplasty cases (32 hips, 13 knees, and 1 unicondylar knee) were studied by taking cultures of sterile splash basins as soon as they are opened (controls) and again at wound closure after instruments and debris have come into contact with the sterile water. All cultures were taken with sterile culture swabs and sent to the laboratory for aerobic, anaerobic, and fungal culture. Outcome measured was any positive culture. A total of 92 cultures from 46 cases were tested. Only 1 (2.17%) control culture, which grew Streptococcus viridans, was positive for bacterial growth. One of 46 samples (2.17%) taken at wound closure was positive for coagulase-negative Staphylococcus. Mean time between basin opening and wound closure was 180±45 minutes. For the 1 infected sample taken at the conclusion of the case, it was 240 minutes. Previous studies show contamination rates as high as 74% for splash basins used intraoperatively. Our study contradicts the belief that splash basins are a high source of infection, with only 2.17% of basins showing contamination. Splash basins can be a potential source of contamination, but the risk is not as high as previously cited in the orthopedic literature.
Insights
Intraoperative splash basins in arthroplasty have a low contamination risk (2.17%), contradicting previous high infection rate beliefs. This study found minimal bacterial growth, suggesting splash basins are not a significant source of infection.
Area of Science:
- Orthopedic Surgery
- Infection Control
- Microbiology
Background:
- Splash basins are utilized in arthroplasty for instrument cleaning.
- Previous literature suggests splash basins may harbor bacteria, posing an infection risk.
Purpose of the Study:
- To evaluate the contamination risk of intraoperative splash basins during arthroplasty procedures.
- To compare current contamination rates with previously reported findings.
Main Methods:
- Cultures were taken from sterile splash basins before and after use in 46 primary arthroplasty cases.
- Samples were analyzed for aerobic, anaerobic, and fungal growth.
- Contamination was defined as any positive bacterial culture.
Main Results:
- Only one control culture (2.17%) showed bacterial growth (Streptococcus viridans).
- One sample (2.17%) taken at wound closure tested positive for coagulase-negative Staphylococcus.
- The mean time from basin opening to wound closure was 180±45 minutes.
Conclusions:
- Intraoperative splash basins demonstrate a low contamination rate (2.17%) in this study.
- Findings challenge the notion that splash basins are a high-risk source of infection in arthroplasty.
- While a potential source, the infection risk from splash basins appears lower than previously suggested.
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