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Related Concept Videos

DNA Replication02:40

DNA Replication

DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication uses a large number of...
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Prokaryotes01:32

Replication in Prokaryotes

DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...

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Related Experiment Video

Updated: May 29, 2026

Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae
08:40

Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae

Published on: October 21, 2022

Dynamics of DNA replication in yeast.

Renata Retkute1, Conrad A Nieduszynski, Alessandro de Moura

  • 1Centre for Genetics and Genomics, University of Nottingham, United Kingdom.

Physical Review Letters
|September 10, 2011
PubMed
Summary

We developed a mathematical model for DNA replication dynamics. Our findings show that mean replication time curves alone cannot determine origin parameters, highlighting the importance of stochasticity in replication timing.

Area of Science:

  • Genetics
  • Molecular Biology
  • Biophysics

Background:

  • DNA replication is a fundamental process for cell division.
  • Understanding the timing and regulation of DNA replication is crucial for genome stability.
  • Previous models often simplified the stochastic nature of replication.

Purpose of the Study:

  • To develop a mathematical model for the spatial dynamics of DNA replication.
  • To determine the probability distribution of replication times for chromosomal positions.
  • To investigate the relationship between replication timing and origin parameters.

Main Methods:

  • Development of a novel mathematical model for DNA replication.
  • Analysis of probability distributions for chromosomal replication times.

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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
07:18

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

Related Experiment Videos

Last Updated: May 29, 2026

Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae
08:40

Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae

Published on: October 21, 2022

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
07:18

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

  • Comparison of model predictions with experimental data from Saccharomyces cerevisiae.

    • Main Results:

    • Mean replication time curves are insufficient for directly determining DNA replication origin parameters.
    • The stochastic nature of DNA replication leaves a detectable signature in population-averaged experimental data.
    • The width of the activation time probability distribution can be inferred from experimental measurements.

    Conclusions:

    • Stochastic processes significantly influence DNA replication timing.
    • New modeling approaches are needed to accurately interpret replication timing data.
    • Our model provides a framework for understanding replication dynamics and inferring key parameters from experimental data.