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Related Experiment Video

Updated: May 29, 2026

Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development
09:43

Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

Published on: August 10, 2015

Renal tissue engineering with decellularized rhesus monkey kidneys: age-related differences.

Karina H Nakayama1, Cynthia A Batchelder, Chang I Lee

  • 1Center of Excellence in Translational Human Stem Cell Research, California National Primate Research Center, University of California, Davis, California 95616-8542, USA.

Tissue Engineering. Part A
|September 10, 2011
PubMed
Summary

Kidney tissue engineering shows promise for new therapies. Donor age significantly impacts the success of repopulating decellularized kidney scaffolds, with younger donors yielding better results for kidney regeneration.

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Related Experiment Videos

Last Updated: May 29, 2026

Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development
09:43

Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

Published on: August 10, 2015

Preparation of Decellularized Kidney Scaffolds in Rats
06:09

Preparation of Decellularized Kidney Scaffolds in Rats

Published on: March 18, 2021

Microdissection of Primary Renal Tissue Segments and Incorporation with Novel Scaffold-free Construct Technology
09:00

Microdissection of Primary Renal Tissue Segments and Incorporation with Novel Scaffold-free Construct Technology

Published on: March 27, 2018

Area of Science:

  • Regenerative Medicine
  • Nephrology
  • Biomaterials

Background:

  • Limited regenerative capacity of kidneys and organ donor shortages necessitate novel therapeutic strategies.
  • Developing functional kidney tissue requires effective methods for cellular repopulation of scaffolds.

Purpose of the Study:

  • To repopulate decellularized kidney scaffolds in vitro.
  • To investigate the influence of donor age on kidney scaffold recellularization efficiency.

Main Methods:

  • Generated decellularized kidney scaffolds from fetal, juvenile, and adult rhesus monkey kidneys, preserving extracellular matrix (ECM).
  • Recellularized scaffolds using fetal, juvenile, and adult renal cell explants and fetal renal cell fractions.
  • Assessed cell infiltration, organization, and tubular structure formation within scaffolds.

Main Results:

  • Successful infiltration and organization of renal cells (vimentin+, cytokeratin+, calbindin+) within kidney scaffolds.
  • Greatest cellular repopulation observed with scaffolds from younger donors (fetal and juvenile).
  • Seeding with mixed fetal renal aggregates promoted tubular structure formation within scaffolds.

Conclusions:

  • Decellularized kidney scaffolds can be effectively repopulated with donor cells.
  • Donor age is a critical factor influencing the efficiency of kidney scaffold recellularization.
  • These findings support the potential of age-matched donor tissues for kidney tissue engineering.