Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fast Reactions01:27

Fast Reactions

Fast reactions occurring in times shorter than the time needed to mix reactants pose a unique challenge for investigation. In a liquid-phase continuous-flow system, reactants A and B are swiftly pushed into the mixing chamber, where mixing occurs within 1 ms. The reaction mixture then flows through an observation tube, and one measures light absorption to determine species concentrations at various points of the tube. This method is most appropriate when relatively large volumes of reactants...
Measuring Reaction Rates03:09

Measuring Reaction Rates

Polarimetry finds application in chemical kinetics to measure the concentration and reaction kinetics of optically active substances during a chemical reaction. Optically active substances have the capability of rotating the plane of polarization of linearly polarized light passing through them—a feature called optical rotation. Optical activity is attributed to the molecular structure of substances. Normal monochromatic light is unpolarized and possesses oscillations of the electrical field in...
Enzyme Kinetics01:19

Enzyme Kinetics

Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
Predicting Reaction Outcomes02:24

Predicting Reaction Outcomes

Kinetics describes the rate and path by which a reaction occurs. In contrast, thermodynamics deals with state functions and describes the properties, behavior, and components of a system. It is not concerned with the path taken by the process and cannot address the rate at which a reaction occurs. Although it does provide information about what can happen during a reaction process, it does not describe the detailed steps of what appears on an atomic or a molecular level. On the other hand,...
Introduction to Enzyme Kinetics01:19

Introduction to Enzyme Kinetics

Enzyme kinetics studies the rates of biochemical reactions. Scientists monitor the reaction rates for a particular enzymatic reaction at various substrate concentrations. Additional trials with inhibitors or other molecules that affect the reaction rate may also be performed.
The experimenter can then plot the initial reaction rate or velocity (Vo) of a given trial against the substrate concentration ([S]) to obtain a graph of the reaction properties. For many enzymatic reactions involving a...
Methods of Medium Optimization01:28

Methods of Medium Optimization

Optimizing growth media enhances microbial proliferation and maximizes product yield. Statistical experimental design methodologies provide structured and reproducible approaches, offering progressively higher levels of robustness and efficiency.The One-Factor-at-a-Time (OFAT) MethodThe One-Factor-at-a-Time (OFAT) method involves adjusting a single variable while keeping all others constant. However, it cannot detect interactions between variables, often leading to suboptimal outcomes when...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same journal

An herbal formula alleviates hyperuricemia: Evidence from network pharmacology and in vivo experiments.

Combinatorial chemistry & high throughput screening·2026
Same journal

Corrigendum to: Jeduxiaoliu Formula can Induce Apoptosis of Lymphoma Cells <i>In Vitro</i> and <i>In Vivo</i>.

Combinatorial chemistry & high throughput screening·2026
Same journal

Calcitonin Improved Chondrocytes in Osteoarthritis through the Wnt Signaling Pathway.

Combinatorial chemistry & high throughput screening·2026
Same journal

Artificial Intelligence Methods for Biomedical, Biochemical, and Bioinformatics Problems.

Combinatorial chemistry & high throughput screening·2026
Same journal

Mechanisms for Anti-Inflammatory Activity of Gold Nanoparticles.

Combinatorial chemistry & high throughput screening·2026
Same journal

Exploring Spectral Graph Theory in Combinatorial Chemistry.

Combinatorial chemistry & high throughput screening·2026

Related Experiment Video

Updated: May 29, 2026

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
06:52

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

Implementation of high throughput experimentation techniques for kinetic reaction testing.

Anton J Nagy1

  • 1ILS-Integrated Lab Solutions GmbH, Oranienstrasse 10-11, Aufgang 1, D-10997 Berlin, Germany. Anton.nagy@integratedlabsolutions.com

Combinatorial Chemistry & High Throughput Screening
|September 10, 2011
PubMed
Summary

High throughput experimentation (EE) tools accelerate the acquisition of reaction kinetic data. These systems are crucial for R&D labs, enabling faster, high-quality kinetic modeling of complex chemical reactions.

More Related Videos

Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions
13:00

Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions

Published on: April 4, 2014

Identification of Kinase-substrate Pairs Using High Throughput Screening
11:13

Identification of Kinase-substrate Pairs Using High Throughput Screening

Published on: August 29, 2015

Related Experiment Videos

Last Updated: May 29, 2026

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
06:52

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions
13:00

Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions

Published on: April 4, 2014

Identification of Kinase-substrate Pairs Using High Throughput Screening
11:13

Identification of Kinase-substrate Pairs Using High Throughput Screening

Published on: August 29, 2015

Area of Science:

  • Chemical Engineering
  • Reaction Kinetics
  • Computational Chemistry

Background:

  • High throughput experimentation (EE) is increasingly adopted in chemical, petrochemical, and polymer R&D.
  • Traditional methods for kinetic data acquisition can be time-consuming.
  • Accurate kinetic data is vital for process optimization and prediction.

Purpose of the Study:

  • To showcase successful implementations of EE systems for kinetic studies.
  • To demonstrate the benefits of EE in deriving reaction kinetic data.
  • To highlight the role of EE in understanding complex reaction mechanisms.

Main Methods:

  • Design and implementation of specific EE systems for kinetic studies.
  • Application of EE to both catalytic and non-catalytic reaction systems.
  • Focus on generating data for kinetic modeling.

Main Results:

  • Demonstrated successful application of EE systems in various R&D settings.
  • Achieved significantly faster acquisition of kinetic data compared to conventional methods.
  • Generated high-quality data suitable for kinetic modeling.

Conclusions:

  • EE tools are essential for modern chemical R&D laboratories.
  • EE significantly enhances the efficiency of kinetic data acquisition.
  • EE facilitates the quantitative prediction of complex, multistep reaction behaviors.