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A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

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Gene Composer in a structural genomics environment.

Don Lorimer¹, Amy Raymond, Mark Mixon

¹Emerald BioStructures, 7869 NE Day Road West, Bainbridge Island, WA 98110, USA. dlorimer@embios.com

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications

|September 10, 2011

Summary

Gene Composer software streamlines high-throughput protein structure determination by aiding in the design of protein and gene sequences. This tool enhances efficiency for researchers in structural genomics and protein engineering.

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Area of Science:

Structural biology
Bioinformatics
Molecular biology

Background:

Structural genomics projects require efficient manipulation of genetic and protein sequences.
High-throughput protein structure determination is crucial for understanding disease mechanisms.

Purpose of the Study:

To develop a database software package, Gene Composer, to enhance efficiency in structural genomics.
To facilitate the design of protein constructs and their underlying gene sequences for high-throughput studies.

Main Methods:

Development of a modular database software package named Gene Composer.
Implementation of graphical user interfaces for bioinformatics workflows.
Application in structure-guided protein engineering and synthetic gene engineering.

Main Results:

Gene Composer facilitates information-rich design of protein and gene sequences.
The software supports common bioinformatics steps in protein engineering.
Successful application demonstrated in H1N1 RNA-dependent RNA polymerase PB2 subunit structure determination.

Conclusions:

Gene Composer improves efficiency in high-throughput protein structure determination.
The software empowers researchers in modern structure-guided protein and synthetic gene engineering.