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Related Experiment Video

Updated: May 29, 2026

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter
10:08

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

Published on: May 21, 2021

The Protein Maker: an automated system for high-throughput parallel purification.

Eric R Smith1, Darren W Begley, Vanessa Anderson

  • 1Seattle Structural Genomics Center for Infectious Disease (http://www.ssgcid.org), USA.

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
|September 10, 2011
PubMed
Summary

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The Protein Maker system automates high-throughput protein purification, enabling structural studies of influenza proteins. This automated system aids in determining protein structures for infectious disease research.

Area of Science:

  • Structural Biology
  • Biochemistry
  • Infectious Disease Research

Background:

  • High-throughput protein purification is essential for structural biology and drug discovery.
  • Automated systems can streamline the complex process of protein purification.
  • The Seattle Structural Genomics Center for Infectious Disease (SSGCID) requires efficient tools for protein analysis.

Purpose of the Study:

  • To evaluate the utility of the automated Protein Maker purification system.
  • To demonstrate the system's capability in purifying recombinant proteins for structural studies.
  • To showcase the application of the Protein Maker in infectious disease research.

Main Methods:

  • Utilized the Protein Maker automated purification system for parallel purification of 24 samples.

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Last Updated: May 29, 2026

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  • Purified five recombinant PB2 C-terminal domains from influenza A virus subtypes.
  • Employed methods for screening lysis buffers for fungal cytochrome P450.

    • Main Results:

    • Successfully purified multiple recombinant proteins, including influenza PB2 domains.
    • Three purified protein constructs were crystallized.
    • One protein structure was determined to sufficient resolution for deposition in the Protein Data Bank.
    • Lysis buffer screening methods were established for fungal cytochrome P450.

    Conclusions:

    • The Protein Maker system is a valuable tool for high-throughput protein purification.
    • The system facilitates structural determination of proteins relevant to infectious diseases.
    • Automated purification accelerates research in structural genomics and infectious disease research.