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GABA transport in Saccharomyces cerevisiae
J McKelvey1, R Rai, T G Cooper
1Department of Microbiology and Immunology, University of Tennessee, Memphis 38163.
Yeast (Chichester, England)
|May 1, 1990
Summary
Saccharomyces cerevisiae accumulates gamma-aminobutyrate (GABA) via an active transport system. Most intracellular GABA may be stored in the vacuole, influenced by nitrogen sources.
Area of Science:
- Microbiology
- Biochemistry
- Cell Biology
Background:
- Gamma-aminobutyrate (GABA) is a key neurotransmitter and metabolite.
- Understanding GABA transport in yeast is crucial for cellular metabolism studies.
Purpose of the Study:
- To investigate the mechanism of gamma-aminobutyrate (GABA) accumulation in Saccharomyces cerevisiae.
- To identify factors regulating GABA uptake and storage in yeast cells.
Main Methods:
- Studied GABA transport using active transport inhibitors and varying pH conditions.
- Analyzed GABA efflux and sequestration using proton ionophores and nystatin.
- Examined the influence of different nitrogen sources on GABA uptake.
Main Results:
- GABA accumulation occurs via an active transport system inhibited by specific ions and protonophores.
- Optimal transport occurs at pH 5.0 with specific Km values.
- Intracellular GABA is largely sequestered in the vacuole, with limited efflux.
- GABA uptake is regulated by nitrogen availability.
Conclusions:
- Saccharomyces cerevisiae possesses a regulated, active transport system for GABA accumulation.
- The yeast vacuole likely serves as the primary storage site for intracellular GABA.
- Nitrogen metabolism significantly influences GABA transport dynamics in yeast.