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Protein kinases in developing rat brain.

S M Lohmann, U Walter, P Greengard

    Journal of Cyclic Nucleotide Research
    |December 1, 1978
    PubMed
    Summary
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    Cyclic adenosine monophosphate (cAMP)-dependent protein kinase levels, including regulatory (R) and catalytic (C) subunits, remained stable during rat brain development. The ratio of R to C subunits was consistently one in both newborn and adult rats.

    Area of Science:

    • Neuroscience
    • Molecular Biology
    • Biochemistry

    Background:

    • Cyclic adenosine monophosphate (cAMP) plays a crucial role in intracellular signaling pathways.
    • cAMP-dependent protein kinases (PKA) are key effectors of cAMP signaling, regulating numerous cellular processes.
    • Understanding the developmental regulation of PKA is essential for comprehending brain maturation and function.

    Purpose of the Study:

    • To quantify the levels of regulatory (R) and catalytic (C) subunits of cAMP-dependent protein kinases in the developing rat brain.
    • To investigate developmental changes in the ratio of R and C subunits.
    • To characterize the abundance of different PKA iso-subunits (Type I and Type II) during brain development.

    Main Methods:

    • Measurement of regulatory subunit (R) levels using [3H]cAMP binding and 8-N3-[32P]cAMP incorporation.

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  • Assay of catalytic subunit (C) activity via histone kinase assays.
  • Quantification of protein levels per milligram of total protein.
  • Separation and quantification of R-I and R-II subunits using polyacrylamide-gel electrophoresis.

    • Main Results:

    • Neither regulatory (R) nor catalytic (C) subunit levels showed significant changes in brain membranes or cytosol during development.
    • The ratio of R to C subunits was approximately one in both newborn and adult rats.
    • Levels of R-I and R-II subunits also remained stable in both membrane and cytosol fractions throughout development.

    Conclusions:

    • cAMP-dependent protein kinase subunit levels are tightly regulated and do not undergo significant changes during rat brain development.
    • The balance between regulatory and catalytic subunits, as well as the relative abundance of PKA types, is maintained from birth to adulthood.
    • These findings suggest a stable PKA system supporting mature brain function from an early developmental stage.