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Related Experiment Video

Updated: May 29, 2026

Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions

Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions

Published on: June 7, 2020

Insights into the Sigma-1 receptor chaperone's cellular functions: a microarray report.

Shang-Yi Tsai¹, Richard Kyle Rothman, Tsung-Ping Su

¹Cellular Pathobiology Section, Integrative Neuroscience Branch, National Institute on Drug Abuse, NIH, DHHS, Baltimore, Maryland 21224, USA.

Synapse (New York, N.Y.)

|September 10, 2011

Summary

Sigmoid-1 receptors (Sig-1Rs) are crucial for neuronal development and function. Their knockdown disrupts cellular processes, impacting pathways linked to neurodegenerative diseases like Alzheimer's and Parkinson's.

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Published on: September 2, 2019

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In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells

In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells

Published on: September 2, 2019

Area of Science:

Neuroscience
Molecular Biology
Cellular Biology

Background:

Sigmoid-1 receptors (Sig-1Rs) are known regulators of neuronal morphogenesis, development, oxidative stress, and mitochondrial function.
Previous studies established the critical role of Sig-1Rs in neuronal health.

Purpose of the Study:

To identify specific pathways and genes influenced by Sig-1R activity.
To investigate the functional consequences of Sig-1R knockdown in mature neurons.

Main Methods:

Gene expression profiling using a rat genome cDNA array.
Adeno-associated virus (AAV) mediated small interfering RNA (siRNA) targeting Sig-1R in rat hippocampal neurons cultured for 18 days in vitro (DIV).
Analysis of cellular functions including steroid biogenesis, protein ubiquitination, actin cytoskeleton, and oxidative stress.

Main Results:

Sig-1R knockdown significantly altered genes involved in actin polymerization, synapse plasticity, steroid biogenesis, protein ubiquitination, and Nrf-2 mediated oxidative stress.
Reduced levels of cytochrome c and increased levels of free-radical generating enzymes (cytochrome p450, cytochrome b-245) were observed in Sig-1R knockdown neurons.
Gene array data suggest Sig-1R involvement in the pathogenesis of central nervous system (CNS) diseases such as Alzheimer's and Parkinson's disease.

Conclusions:

Sig-1Rs play a critical role in maintaining cellular functions within the CNS.
Disruption of Sig-1R function impacts pathways relevant to neurodegenerative disorders.
These findings support Sig-1Rs as potential therapeutic targets for CNS diseases.