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Updated: May 29, 2026

CRISPR-based Shuttle Cloning: A High-throughput Cloning Method
Published on: June 13, 2025
A rapid and cost-effective method for sequencing pooled cDNA clones by using a combination of transposon insertion
Takeya Morozumi1, Daisuke Toki, Tomoko Eguchi-Ogawa
1Animal Genome Research Program, Animal Research Division, Institute of Society for Techno-innovation of Agriculture, Forestry and Fisheries, Tsukuba, Japan.
Abstract:
Large-scale cDNA-sequencing projects require an efficient strategy for mass sequencing. Here we describe a method for sequencing pooled cDNA clones using a combination of transposon insertion and Gateway technology. Our method reduces the number of shotgun clones that are unsuitable for reconstruction of cDNA sequences, and has the advantage of reducing the total costs of the sequencing project.
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