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Published on: May 16, 2025
[Combination of AD5-10 and epirubicin in treating rheumatoid arthritis]
Jian-suo Zhou1, Juan Shi, Jie-qing Zhu
1National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Beijing, China.
Objective:
To investigate the mechanism of anti-death receptor 5-10 (AD5-10) combined with epirubicin in treating rheumatoid arthritis (RA).
Methods:
We detected the cell viability of the fibroblast-like synoviocytes (FLS) from RA patients with MTT. The expression level of apoptosis signaling pathways protein, p53, and p21 were evaluated with Western blot.
Results:
We found that epirubicin, at different doses, could enhance the effect of AD5-10 on FLS, promoting the apoptosis of FLS. The expression levels of caspase-3, -8, -9, c-FLIP, Bcl-2, p53, and p21 in the FLS changed after epirubicin treatment.
Conclusion:
Epirubicin may coordinate with AD5-10 in inducing FLS apoptosis through affecting the levels of p53, p21, c-FLIP, and Bcl-2.
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