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Published on: September 19, 2016
[TLR4 expression of human PBMC treated by BCG and its role of immune activation]
Rui Sun1, Xiong Yang, Er-Lin Sun
1Based Medical Research Center, Tianjin Medical University, Tianjin 300070, China.
Aim:
To investigate TLR4 expression of human peripheral blood mononuclear cells (hPBMC) treated with BCG and its role of immune activation.
Methods:
hPBMC were treated with BCG in vitro. TLR4 expression were detected by flow cytometry, IFN-γ and TNF-α expression of hPBMC in both BCG stimulated group and the control group were detected by ELISA.
Results:
The expression of TLR4 in hPBMC treated with BCG was stronger than the control group significantly (P<0.01) and increased with the time. In 72 h the TLR4 expression of BCG group was (44.73 ± 0.0066)%, while the control group was (1.02 ± 0.0024)%. BCG can promote hPBMC proliferation, and this enhancement was time-dependent. In 24 h, 48 h and 72 h IFN-γ and TNF-α expression of BCG group were significantly higher than the control group(P<0.05), and this enhancement was time-dependent.
Conclusion:
BCG can on enhance TLR4 expression and promote immune activation of hPBMC.
