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Related Concept Videos

Plasmids01:28

Plasmids

Plasmids are extrachromosomal DNA molecules found in bacteria, archaea, and some eukaryotic microbes like yeast. These small, circular DNA structures typically contain fewer than 30 genes, although some may exist linearly. Plasmids vary in their number within a cell, known as copy number. Single-copy plasmids are present in one copy per cell and multi-copy plasmids are present in multiple copies, reaching over 100 copies per cell.Plasmids usually replicate independently of the chromosomal DNA...
DNA Bacteriophages01:26

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...

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Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli
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Sequence analysis and characterization of two cryptic plasmids derived from Lactobacillus buchneri CD034.

Stefan Heinl1, Katharina Spath, Esther Egger

  • 1Christian-Doppler-Laboratory for Genetically Engineered Lactic Acid Bacteria, Department of Biotechnology, University of Natural Resources and Life Sciences, VIBT, Muthgasse 11, 1190 Vienna, Austria. stefan.heinl@boku.ac.at

Plasmid
|September 13, 2011
PubMed
Summary

Two novel plasmids from Lactobacillus buchneri were sequenced and characterized for the first time. These plasmids are valuable for developing new shuttle and expression vectors for lactic acid bacteria (LAB).

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Biotechnology

Background:

  • Lactobacillus buchneri is crucial for silage preservation due to its acid resistance and robustness.
  • Its potential for silage starter cultures and genetic engineering warrants further investigation.

Purpose of the Study:

  • To isolate, sequence, and characterize two cryptic plasmids from Lactobacillus buchneri CD034.
  • To assess the potential of these plasmids for developing novel vectors for lactic acid bacteria (LAB).

Main Methods:

  • Plasmid isolation, DNA sequencing, and bioinformatic analysis.
  • Identification of open reading frames (ORFs), replication proteins, and mobilization proteins.
  • Construction and testing of shuttle vectors in other bacterial species.

Main Results:

  • Two plasmids, pCD034-1 (3424 bp) and pCD034-2 (2707 bp), were fully characterized.
  • Both plasmids contain predicted ORFs encoding replication and mobilization proteins, with similarities to known plasmids.
  • Shuttle vectors derived from these plasmids were successfully constructed and used for transformation in other LAB species.

Conclusions:

  • The characterized plasmids represent a novel resource for Lactobacillus buchneri.
  • These plasmids can be utilized to generate versatile shuttle and expression vectors for LAB, advancing genetic engineering applications.