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DNA as a Genetic Template

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...

DNA as a Genetic Template

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

DNA Isolation

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DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Although all next-generation methods use different technologies, they all share a set of standard features.

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Related Experiment Video

Updated: May 29, 2026

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

Generating STR profile from "Touch DNA".

Sangeeta Aditya¹, A K Sharma, C N Bhattacharyya

¹Central Forensic Science Laboratory, 30 Gorachand Road, Kolkata 700014, India. sangadt@gmail.com

Journal of Forensic and Legal Medicine

|September 13, 2011

Summary

Scientists can now profile touch DNA from skin cells left on items. This study uses low-copy number (LCN) typing and miniSTRs to enhance DNA analysis for criminal investigations.

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Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

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Area of Science:

Forensic Science
Genetics
Molecular Biology

Background:

Forensic DNA technology is crucial in criminal investigations.
Obtaining DNA from touch evidence (skin cells) presents challenges.
Advancements are needed to analyze trace DNA effectively.

Purpose of the Study:

To evaluate the efficacy of combining Low-Copy Number (LCN) typing and miniSTRs for touch DNA analysis.
To demonstrate the value of this combined technology for profiling trace DNA evidence.

Main Methods:

DNA profiling of touched evidence materials was performed.
A combination of LCN typing and miniSTR technology was employed.
Analysis focused on DNA recovered from skin cells left by contact.

Main Results:

Successful DNA profiling was achieved from touched evidence materials.
The combined LCN typing and miniSTR approach proved effective for trace DNA.
The technology expands the scope of DNA profiling for numerous touched items.

Conclusions:

The combination of LCN typing and miniSTRs is a valuable tool for touch DNA analysis.
This methodology enhances the ability to obtain DNA profiles from challenging forensic samples.
The technology broadens the application of DNA profiling in criminal investigations.