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Related Experiment Video

Updated: May 29, 2026

Semi-automated Analysis of Mouse Skeletal Muscle Morphology and Fiber-type Composition
08:36

Semi-automated Analysis of Mouse Skeletal Muscle Morphology and Fiber-type Composition

Published on: August 31, 2017

Automated multiscale morphometry of muscle disease from second harmonic generation microscopy using tensor-based

Christoph S Garbe1, Andreas Buttgereit, Sebastian Schürmann

  • 1Interdisciplinary Center for Scientific Computing, University of Heidelberg, 69115 Heidelberg, Germany. Christoph.Garbe@uni-heidelberg.de

IEEE Transactions on Bio-Medical Engineering
|September 13, 2011
PubMed
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This study introduces an automated imaging analysis to detect tissue remodeling in Duchenne muscular dystrophy (mdx mouse) models. The method quantifies morphological changes, aiding in diagnosing and monitoring chronic muscle diseases.

Area of Science:

  • Biomedical Engineering
  • Cellular and Molecular Biology
  • Pathology

Background:

  • Chronic diseases involve tissue remodeling, altering organ and cellular function.
  • Morphological changes can become irreversible, driving disease progression.
  • Early diagnostics and monitoring of tissue alterations require advanced imaging.

Purpose of the Study:

  • To develop and validate an automated image processing approach for quantifying morphological changes in muscle tissue.
  • To assess the utility of this method in an animal model of Duchenne muscular dystrophy (mdx mouse).
  • To establish a prerequisite for clinical image databases for diagnosing and monitoring chronic muscle diseases.

Main Methods:

  • Combined second harmonic generation (SHG) microscopy and automated image processing.

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3D Ultrasound Imaging: Fast and Cost-effective Morphometry of Musculoskeletal Tissue
08:52

3D Ultrasound Imaging: Fast and Cost-effective Morphometry of Musculoskeletal Tissue

Published on: November 27, 2017

Related Experiment Videos

Last Updated: May 29, 2026

Semi-automated Analysis of Mouse Skeletal Muscle Morphology and Fiber-type Composition
08:36

Semi-automated Analysis of Mouse Skeletal Muscle Morphology and Fiber-type Composition

Published on: August 31, 2017

3D Ultrasound Imaging: Fast and Cost-effective Morphometry of Musculoskeletal Tissue
08:52

3D Ultrasound Imaging: Fast and Cost-effective Morphometry of Musculoskeletal Tissue

Published on: November 27, 2017

  • Acquisition of multiphoton XYZ image stacks from mdx mouse and healthy control tissue slices.
  • Adaptation and optimization of the boundary-tensor approach for SHG data analysis.

    • Main Results:

    • Significant morphological deviations were observed in muscles of old mdx mice, including irregular cell calibers, twisted myofibrils, and larger sarcomere lattice disruptions.
    • Young mdx mice showed only minor alterations.
    • The automated algorithm demonstrated comparable reliability to manual detection but with significantly improved speed.

    Conclusions:

    • The developed SHG microscopy and automated image processing approach effectively quantifies morphological alterations in muscle tissue.
    • This method is suitable for rapid, quantitative morphometry and shows promise for clinical applications in diagnosing and monitoring chronic muscle diseases.
    • The boundary-tensor approach provides a reliable tool for analyzing SHG data, outperforming manual methods in efficiency.