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Updated: May 29, 2026

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Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins
Published on: July 1, 2011
Determination of Borrelia surface lipoprotein anchor topology by surface proteolysis
Shiyong Chen1, Ozan S Kumru, Wolfram R Zückert
1Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Mail Stop 3029, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA.
Journal of Bacteriology
|September 13, 2011
Abstract:
We used a surface trypsinolysis assay to probe accessibility of the membrane-proximal N-terminal tether peptides of Borrelia surface lipoproteins OspA and Vsp1. Our findings with both wild-type and mutant proteins are only compatible with the anchoring of these surface lipoproteins in the outer leaflet of the outer spirochetal membrane.

