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Updated: May 29, 2026

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone
Published on: February 23, 2017
Counter-ionic effect on the separation of water-soluble compounds applying a hydrophilic stationary phase bonded with
Waka Kamichatani1, Yoshinori Inoue, Mitsuru Saito
1Nippon Filcon Co., Ltd., 2220 Ohmaru, Inagi, Tokyo 208-8577, Japan. w-kamichatani@filcon.co.jp
Abstract:
A novel hydrophilic stationary phase bonded with a zwitter-ionic polymer for HPLC was synthesized. The stationary phase, in combination with a mobile phase containing various salts, was evaluated for its ability to separate water-soluble compounds, such as nucleobases, nucleosides and glycosides. The retention of a large majority of the solutes, except for cytosine, was increased by adding anti-chaotropic ions to the mobile phase. These results suggested that the retention of solutes depended on the thickness of the hydration layer on the stationary phase. In the zwitter-ionic polymer adsorbent, the formation of the hydration layer and the ionicity of the zwitter-ionic group on the stationary phase will be controlled by the properties of the ions added to the mobile phase.
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