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Published on: October 21, 2013
Establishment of a confluent cardiomyocyte culture in a cylindrical microchannel
Yo Tanaka1, Hiroto Akaike, Yasuhiko Sugii
1Department of Applied Chemistry, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8656, Japan.
Abstract:
We established a confluent cardiomyocyte culture method using an 800-µm diameter cylindrical microchannel in this report. This was realized by introducing cardiomyocytes 2 times before and after turning over a microchip. The optimum condition was starting the flowing medium 2.0 h after seeding and flowing the medium at 1.0 µL/min. By applying this technology to a cardiomyocyte-based spherical heart pump device, one may develop self-fluid regulated devices that could be applied for implantable or circulation analysis device on a chip.
