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Hydrocarbon productivities in different Botryococcus strains: comparative methods in product quantification.

Ela Eroglu, Shigeru Okada, Anastasios Melis

Journal of Applied Phycology

|September 13, 2011

Summary

This study compared hydrocarbon production in six Botryococcus microalgae strains. B-race strains showed higher hydrocarbon accumulation, validating methods for selecting high-yield algae for commercial use.

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Area of Science:

Biotechnology
Microalgal Cultivation
Biorefinery

Background:

Botryococcus is a microalga known for hydrocarbon production.
A-race and B-race strains accumulate different hydrocarbon types (alkadienes and botryococcene).
Efficient methods are needed to quantify hydrocarbon content for strain selection.

Purpose of the Study:

To compare biomass and hydrocarbon productivities of six different Botryococcus strains.
To validate analytical methods for hydrocarbon quantitation.
To identify high-yield microalgae strains for commercial applications.

Main Methods:

Gravimetric assessment of biomass productivity under laboratory conditions.
Hydrocarbon productivity measured using density equilibrium, spectrophotometric analysis, and gravimetric quantitation.
Comparison of results from three independent hydrocarbon quantification methods.

Main Results:

B-race strains (Botryococcus braunii var. Showa and Kawaguchi-1) accumulated 30% and 20% botryococcene hydrocarbons, respectively.
A-race strains (Botryococcus braunii varieties Yamanaka, UTEX 2441, UTEX LB572) accumulated 14% to 10% alkadiene hydrocarbons.
Botryococcus sudeticus (UTEX 2629) showed the lowest accumulation (approx. 3%).

Conclusions:

Density equilibrium and spectrophotometric methods are validated for botryococcene quantification.
Significant differences in hydrocarbon accumulation exist between A-race and B-race Botryococcus strains.
These validated methods will aid in screening microalgae for high hydrocarbon content for commercial use.